Using the NK Cell Isolation Kit, human, NK cells are isolated by depletion of non-target cells. Non-target cells are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens and a cocktail of MicroBeads. In between the two labeling steps no washing steps are required. The
magnetically labeled non-target cells are depleted by retaining them within a MACS?Column in the magnetic field of a MACS Separator, while the unlabeled NK cells run through the column.